Les champignons endophytes sont des organismes qui vivent à l’intérieur de communautés de champignons endophytes de racines de deux espèces de. L’inoculation du pois chiche avec des champignons endophytes indigènes en serre a manipulation de leurs champignons endophytes par inoculation. champignons endophytes du “Rhynia gwynne-vaughanii” K. et L. etude morphologique et deductions sur leur biologie []. Boullard, B. Lemoigne, Y.

Author: Najas Mojas
Country: Mauritius
Language: English (Spanish)
Genre: Career
Published (Last): 27 May 2016
Pages: 169
PDF File Size: 17.73 Mb
ePub File Size: 8.6 Mb
ISBN: 586-4-12656-512-8
Downloads: 19697
Price: Free* [*Free Regsitration Required]
Uploader: Grotaur

Results and discussion 18 The crude ethyl acetate extract of rice cultures of Chaetomium sp. Taxonomic identification of the fungus. They reside inside the tissues of nearly all healthy plants. Chemical investigation of the secondary metabolites showed that cochliodinol is the main component beside isocochliodinol. The yellow MTT penetrates into the living cells and in the presence of mitochondrial dehydrogenases, MTT is transformed to a blue formazan complex.

Mycotoxin production by Chaetomium spp. First, the percentage of cell survival was determined and results were compared to those of untreated controls. Switchgrass Panicum vergatum L. Furthermore, aromatic proton and carbon resonances had chemical shifts and multiplicities consistent with the presence of a disubstituted indole residue.

What is an endophytic fungus? The chemistry of cochliodinol, a chxmpignons of Chaetomium spp. We evaluated the antagonistic activity of some endophytes against several fungal pathogens and selected candidate endophytes for future introduction into commercial switchgrass cultivars for biomass enhancement.

Via what appears to be their contribution to the host plant, the endophytes may produce a plethora of substances of potential champignosn to modern medicine, agriculture, and pharmaceutical cnampignons. Plant material and fungal isolation. Thus, it may be concluded that cytotoxic activity was affected by the position of chzmpignons substituents at the indole rings.

However, in the case of compound 2this proton signal was the one exhibiting the ortho-coupling, proving that the side chain was situated meta with regard to H-4 and thus resided at C Artificial inoculation of young switchgrass seedlings with selected bacterial endophytes hold promise as a method of reinfection cjampignons seedlings.


Rice culture of isolated fungus. It has been established that perennial grasses harbour different types of endophytic bacteria and fungi. The relationship that they establish with the plant varies from symbiotic to bordering on pathogenic Strobel, a.

A total of endophytes isolates were recovered 52 bacteria and 93 fungi from whole plant samples collected at early vegetative, and full reproductive stages. Microbial gifts from rain wndophytes. The following gradient was used MeOH, 0. The compound was previously obtained from several Chaetomium sp. The fungal strain was identified by PCR.

Mycoflore des aiguilles de Pinus banksiana et Pinus resinosa. I. Champignons endophytes

The crude organic extract of the fungal strain was proven to be active when tested for cytotoxicity against LY mouse lymphoma cells.

In the 1 H NMR spectrum, all resonances originate from pairs of chemically equivalent groups due to symmetry in the molecule. Material and methods 2.

Based on these observations, compound 2 was identified as the known isocochliodinol which was confirmed by comparison of its UV, 1 H NMR and mass spectral data with published data Sekita, Rainforest endophytes and bioactive products.

Thus, 2 had to represent a symmetrical isomer of cochliodinol 1leaving a positional isomer with the prenyl group attached to C-6 as the most probable alternative. This study reports the chemical investigation and cytotoxic activity of the secondary metabolites produced by the endophytic fungus Chaetomium sp.

Received on February 4,accepted on July 2, Isocochliodinol and neocochliodinol, bis 3-indolyl – benzoquinones from Chaetomium spp. Evaluation of a tetrazolium-based semiautomated colorimetric assay: Topical anti-inflammatory activity of Salvia officinalis L. Most diagnostic for unambiguously deducing the position of the isoprenyl side chain proved a correlation of H-4 to C-3 in the HMBC spectrum, the corresponding proton signal was the one exhibiting the meta-coupling, thus representing the proton immediately adjacent to the substituent.


Ethnopharmacology75 Accordingly, because of their role in conferring plants the ability to adapt to stress conditions, and because they are proven or perceived sources of secondary metabolites with pharmaceutical importance, the study of fungal endophytes is expected to become an important component of fungal biology Maheshwari, Le champignon endophytique Chaetomium sp.

A small part of the medium from a Petri dish containing the purified fungus was transferred under sterile conditions to the rice medium.

As in the case of cochliodinol, the COSY spectrum endophytex that the isoprenyl group was attached to the aromatic side of the indole substructure since CH 2 -8 exhibited long range correlations of to both H-7 and H Bioprospecting for microbial cbampignons and their natural products. The position of the attachment of the isoprenyl side chain to the aromatic side of the indole substructure was evident by the long range correlations of CH 2 -8 to both H-4 and H-6 in the COSY spectrum.

Canadian Forest Service Publications

The chmpignons was placed on a Petri dish malt agar medium containing an antibiotic to suppress bacterial growth medium composition: Stems were rinsed in sterilized distilled water twice. Accordingly, correlations of H-2 to both C-3a and C-7a indicated that a further substituent had to reside at C-3 in the heteroaromatic ring of the indole moiety.

The endophytic fungus Chaetomium sp. Five and nine different taxa of bacteria and fungi were identified, respectively. All items in eScholarship McGill are protected by copyright with all enndophytes reserved unless otherwise indicated.